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i scei restriction enzyme  (New England Biolabs)


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    Structured Review

    New England Biolabs i scei restriction enzyme
    I Scei Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 657 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/scei+restriction+enzyme/I-SceI/pmc12781945-44-22-25
    Average 97 stars, based on 657 article reviews
    i scei restriction enzyme - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Construct:

    Article Title: ATR and CDK4/6 inhibition target the growth of methotrexate-resistant choriocarcinoma
    Article Snippet: .. Briefly, plasmids were linearised by I-SceI restriction enzyme as follows: 10 μg of reporter construct (NHEJ or HR), 10 μl of 10x CutSmart® Buffer (NEB), 5 μl of I-SceI (25 U) and ddH2O for a total of 100 μl. ..

    Non-Homologous End Joining:

    Article Title: ATR and CDK4/6 inhibition target the growth of methotrexate-resistant choriocarcinoma
    Article Snippet: .. Briefly, plasmids were linearised by I-SceI restriction enzyme as follows: 10 μg of reporter construct (NHEJ or HR), 10 μl of 10x CutSmart® Buffer (NEB), 5 μl of I-SceI (25 U) and ddH2O for a total of 100 μl. ..

    Synthesized:

    Article Title: Transcriptional control of the lacZ promoter is under directional and diversifying selection
    Article Snippet: .. For this purpose we first digested the synthesized insert ( Supplementary File 1 ) from the plasmid on which it was delivered (Twist Biosciences) and the vector pTKDP- neo ( ) using I-SceI restriction enzyme followed by rSAP treatment to avoid self-ligation (New England Biolabs). .. We then ran the digested products on 1% agarose gel and extracted the desired fragments using StrataPrep DNA Gel Extraction Kit (Agilent).

    Plasmid Preparation:

    Article Title: Transcriptional control of the lacZ promoter is under directional and diversifying selection
    Article Snippet: .. For this purpose we first digested the synthesized insert ( Supplementary File 1 ) from the plasmid on which it was delivered (Twist Biosciences) and the vector pTKDP- neo ( ) using I-SceI restriction enzyme followed by rSAP treatment to avoid self-ligation (New England Biolabs). .. We then ran the digested products on 1% agarose gel and extracted the desired fragments using StrataPrep DNA Gel Extraction Kit (Agilent).



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